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Cambridge AS & A Level Biology

3.2Factors that affect enzyme action

Factors that affect enzyme action in Cambridge AS & A Level Biology: what the syllabus expects you to know, the learning objectives and how to practise.
Subtopic40 practice questions2 lessons

Learning objectives

What the syllabus expects you to be able to do.

  1. 3.2.1Investigate and explain the effects of temperature, pH (with buffers), enzyme, substrate and inhibitor concentration on rate.
  2. 3.2.2Explain how Vmax is used to derive Km and how Km compares enzymes' affinity for their substrates.
  3. 3.2.3Explain the effects of competitive and non-competitive reversible inhibitors.
  4. 3.2.4Compare enzymes immobilised in alginate with free enzymes and state the advantages of immobilisation.

Lessons

  • Enzyme inhibitors, Vmax, Km and immobilised enzymes

    40 min

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  • Factors affecting enzyme activity

    35 min

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Sample question

A sample question on this topic. Answer it, check it, then read the worked answer.

Factors that affect enzyme action: sample question

1 mark

Why does the rate of an enzyme-catalysed reaction increase as temperature rises from 10 °C to 30 °C?

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